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nicking reaction catalysed with the MobA89K and MobC89K proteins. The cleaved strand is then unwound through the helicase and sent to the 89K-encoded GI-kind T4SS machinery. The VirB1-89K ingredient is thought to work as a lytic transglycosylase, locally opening the peptidoglycan with contributions from A few other component(s) in the course of the